scale-up of nature’s tissue weaving algorithms to engineer advanced functional materials
We are actually the material of our tissue fabric and its fiber weaving and textile. The arrangement of collagen, elastic protein and other structural proteins in space and time embodies our tissues and organs with amazing elasticity and multi-functional intelligent properties. For example, the mucosa is a soft tissue sleeve that wraps the surface of all the non-joint bones of the body, including an inherent \"smart\" material that gives additional strength to the hard bones under high impact loads. However, there is a lack of scalable bottom The Up method hinders the use of intelligent tissue biology, machinery, and tissue details to create Advanced Functional Materials. Here, a new method is established to amplify multi-dimensional fiber patterns of natural soft tissue for rapid prototyping of Advanced Functional Materials. Second and second harmonics Photon Excitation microscope for drawing microscopic three-dimensional (3D) The arrangement, composition and distribution of collagen and elastic protein fibers of the membrane, and soft tissue sheaths surround all non-joint bone surfaces in our body. Then, expand this natural organizational structure using engineering rendering software, as well as multi-dimensional weaving algorithms, and create macro-organizational prototypes using computers Jacquard loom. The ability of the prototype amplification structure of natural fabrics provides a new way to create Advanced Functional Materials. Sheep research was approved by the Institutional Animal Care and Use Committee (IACUC) It is located in Gerrison, Switzerland. All methods are carried out in accordance with the relevant guidelines and regulations of this IACUC. Visualization and quantification of bone perfusion, 0. Intravenous injection of procion red liquid 8% ( Imperial London chemistry)at a dosage 0. 5 minutes before the implementation of euthanasia, the weight is 01l l/kg. Immediately after euthanasia, the femurs, cartilage membranes and surrounding muscle layers were removed and a standardized protocol was used to prepare a fixed, non-de-mine histology. Using a diamond wire saw, the Poly-a-fat embedded tissue block obtained from transverse cutting per 500 u2009 μm ( Well model 4240). After polishing to 100 μm ( Automatic Network 2000 polishing) , Partially mounted on a slide with a glass cover slide (Eukitt). Link tissue fabric tissue to the general history of mechanical loading, primary and secondary central axes of bone Cross Calculate the section area using macros in image J ( NIH Image J 2 v1. 49). Main and secondary center axes (CA) Cross representative Geometric properties of the section, the most curved crankshaft of the long bone (major CA)and least (minor CA) May resist a given bending load. Previous studies have shown that these reference points can be calculated automatically, reducing the likelihood of bias, while allowing the outcome measurement to be directly related to the load pattern in the age and treatment-matching cohort. Primary and secondary CAs are marked on the outer edge of the bone specimen installed and as high- Resolution microscope (and ). Use the Leica SP5 II inverted microscope equipped with spectral physics MaiTai HP deep-sea titanium sapphire multi-photon laser (~100 fs pulse) , Xyz high-precision multi-point positioning platform and 63 × 1. 3NA target. The second harmonic collagen signal transmitted in the forward direction is not transmittedDescanned- A detector using a 390-440 kbps nm passband filter. The two- Photon Excitation of the elastic protein is stimulated at 830 nm and collected in photos Multiplication tube (PMT) Use a 435-495 nm transmit filter. This filter is used to separate the autofluorescence observed in the green channel that does not fully correspond to the elastic protein structure. For the procion red signal, 580 nm excitation is collected in the PMT using a 650-561 nm emission. Tile scans were collected in the four quadrants associated with the primary and secondary central axes of the 3 channels mentioned earlier, as well as brightfield. Each 246 μm × 246 μm Watt is at 12- The scan speed is 100 hz and the resolution is 2 bits. 081 pixels per Micron. High in tile area Resolution xyz stack with a step size of 0. 5 μm, the size of the body is 0. 48u2009μmu2009×u20090. 48u2009μmu2009×u20090. 50 μm was obtained to capture the collagen, elastic protein and vascular distribution of the sample in three dimensional space (and ). Import tile image and xyz stack to image J2 (NIH picture J2 v149) , Merge to tile overlapping images and converttif file. The sequence of images in Xyz stack is imported into Mimics through a separate channel ( Implementation of MIS Research 18. 0 Beta). The threshold of the shield is determined by the strength of the signal. Two masks are used to distinguish between strong, weak collagen and elastic protein signals. The lower threshold of \"strong mask\" is determined by the minimum intensity required to enable mask with continuous pixels representing sample features. The lower limit of the \"weak mask\" is determined by minimizing the noise in the sample. For rapid prototyping, the mask is converted. Import stl files into STL visualization software (Materialise 3- Study v10. 0 Beta). Remove noise from the model by filtering small shells up to 1 μm and then re-filtering Convert to binary STL file. Then combine these channels together to create a composite model of collagen, elastic protein, and vascular structure, which is completely scalable for subsequent rapid prototyping using a new multi-dimensional weaving algorithm (and ). A recursive method based on natural tissue and fiber is used for prototype fabrics. The yarn fiber composition and stiffness gradient are realized by amplification of natural fiber gradient and spinning yarn. Weaving and high-order architecture using customized computers Auxiliary weaving algorithm (ArahWeave) On the Jaka loom (AVL Looms) Used to fully control each yarn woven into the fabric, as well as post-processing after weaving of the fabric, similar to post-translation modification of the extracellular matrix protein. Nylon single wire woven thread ( Teleflex Medical, Wayne PA) And elastic yarn ( Madeira, Freiburg, Germany) Used to simulate collagen and elastic protein fibers, respectively (). In the case of stretching to physiological displacement, a prototype sample of woven textile was mechanically tested (12u2009mm range) At a constant strain rate (0. 1u2009mm/s)( Bose Corporation, Eden Prairie, MN) electric 3200. In order to generate the strain map, trace the mark on the Canon Legria HF G25 camera during loading (Canon Inc. , TYO, JP). Use the custom cpcorr function in MatLab to calculate the relevant strain map of Digital Imaging (Mathworks Inc. , Natick, MA). In order to test the significance of the difference in elastic modulus between materials, I. Analysis of variance was completed in Minitab 17 (Minitab Inc. State University, PA)().