Levitate cancer cells for rapid 3D tissue
When the magnet is \"suspended\", abundant cells that grow into balls can provide a new way for the laboratory to study cancer and produce alternative tissues for graft use.
Magnets can shape the ball into the shape of tumors and tissues that are similar to growth in the body.
Glauco Souza of Houston nano 3d Bioscience, Texas, and his colleagues used iron oxide and bacterioph-to infect bacteria and bind but not harm viruses to cultivate human mammalian cells from brain tumors.
Phage swallowed up iron oxide, attached them to the cells, and then injected them into magnetic matter.
Next, the researchers put the cells into a Petri dish half full of gel and covered it with a magnetic lid.
The cell response magnet rises from the bottom of the plate and gathers at the interface between the gel and the air.
After 72 hours, the sphere with a diameter of 1mm is formed.
\"Cells like to get in touch with each other,\" Souza said . \".
Compared to cells cultured in 2D, protein expression is closer to real cancer cells in these nerve cell tumors, suggesting that this method can be used to study cancer.
This can also reduce the need for animal testing.
For example, by infecting the brains of mice with human cancer cells, a common, very fatal brain tumor was studied.
This may be avoided by using human cell balls.
The use of this method is not just to study cancer.
These cells are exposed to air and liquid and can be used to simulate the lungs and test drugs for lung diseases.
The team also found that they could make cell balls of different shapes by moving the lid, changing the shape of the lid and the strength of the magnet.
This may allow the skin and long and thin nerve graft to grow in the laboratory.
\"What\'s really cool is that we can draw tissue culture into new shapes,\" said Souza . \".
George Whiteside of Harvard University said one of the key advantages of this approach is speed, he did not participate in the work, but last year discovered a technology to build a 3D culture using stacked paper.
\"The magnetic force brings particles together quickly,\" he said . \"
\"If you test the toxicity of 100,000 drugs on 100,000 tissue samples, it\'s really important to save time, A colleague from souza, Tom Killian from the Department of Physics and astronomy at Rice University in Houston, Texas, added.
However, Whiteside added that Souza\'s team has not yet addressed \"one of the three main issues --
Three dimensional tissue culture \"-cells inside the culture tend to die due to lack of nutrition and oxygen.
\"This technology is a great way to make culture self-conscious.
But it is still plagued by this problem . \"
Killian agreed but said the team was looking for a solution they were not ready to discuss.
Magazine reference and colon;
Natural nanotechnology, DOI and colon cancer. 10. 1038/nnano. 2010.